Basic principles and applications of digital holographic microscopy
نویسندگان
چکیده
Digital holographic microscopy (DHM) is a modern technology allowing three-dimensional (3D) real-time non-destructive marker-free quantitative-phase imaging of inspected samples. DHM ranges from biological and biomedical applications to engineered surfaces metrology. In this Chapter we review the fundamentals of DHM. Starting from a theoretical analysis, we overview the main application fields of DHM. This Chapter introduces the reader in the field of DHM while providing a complete bibliography for a deeper study. As an example of application, we present the analysis of the capabilities provided by DHM when studying a fixed swine sperm biosample.
منابع مشابه
Principles and applications
In digital holography holograms are recorded by a CCD and image reconstruction is performed by a computer. It is free from tedious photographic processing and delivers three-dimensional distributions of both amplitude and phase quantitatively. Its main limitation that is caused by much lower resolution of CCDs than photographic materials has been substantially overcome by phase-shifting digital...
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